6 Steps to Consider for Reducing Pipetting Errors

Charlie Brown By Charlie Brown, 9th Jun 2018 | Follow this author | RSS Feed | Short URL http://nut.bz/2fx8dyks/
Posted in Wikinut>Guides>Science>Chemistry

Precision and accuracy is the key to proper pipetting, especially when you perform it while sitting on the bench. Without precise and accurate pipetting, you cannot determine absolute stock solutions that lead to improper assays, destroying all your efforts that you put into the overall experiment for so long.

6 Steps to Consider for Reducing Pipetting Errors

Precision and accuracy is the key to proper pipetting, especially when you perform it while sitting on the bench. Without precise and accurate pipetting, you cannot determine absolute stock solutions that lead to improper assays, destroying all your efforts that you put into the overall experiment for so long. Having large margin errors will make the entire procedure of pipetting meaningless, something that will make you feel highly irritated.
Even though you can largely avoid mistakes in pipetting by investing in serological pipettes, the accuracy of the results can only be determined by your self-confidence and self-consciousness. With that being said, here are some of the effective solutions and ways through which you can reduce committing pipetting mistakes:

Acknowledge the Importance and Usage of Air Displacement Pipettes

When pipetting, it is crucial to understand how the equipment actually works. Most likely, you will be using an air displacement pipette which works like a syringe. The only difference is that it comes with a cushion filled with air in between the sample and the piston. This air cushion plays a vital role in preventing contamination by keeping the piston away from the solutions. The only thing that affects the pipetting accuracy is the volume of this air cushion, which is determined by pressure and temperature. In case you will be working with volatile solutions or corrosive materials, consider using other types of pipettes.

Wet the Pipette Tip Beforehand

If you are not happy with the outcomes of your lab experiments and looking to increase the accuracy of the pipette, consider pre-wetting the pipette tips prior to using it again. While dispensing liquid from the pipette, mostly there is a coating of that liquid left which hampers the final outcome of the experiment. As the coating in on the tip, dispensing the pipette back to back can ensure removal of the liquid coating and you can have better results.

Reverse Pipetting

Perhaps this is one of the greatest techniques to ensure effective and solid results when pipetting volatile solvents and viscous liquids. All you need to do is, force the piston down and push it back to the second stop or purge position before you get rid of the liquid. The method of reverse pipetting is generally pre-wetted that facilitates the pipetting of volatile solvents.

Consider the Temperature

When manufacturers calibrate the serological pipette, it mostly happens at room temperature. If your work and experiment involve being at different temperatures, there can be huge issues regarding displayed volumes.

Support the Dispensed Volume with Sensible Pipette

In case the dispensed volume reaches the minimum capacity of the pipette, the accuracy of your pipette will be reduced. For avoiding such mistakes, choose the dispensed volume and relevant pipette tips in accordance with each other.

Get Your Pipette Serviced at Fixed Intervals

The pipette servicing is mostly based on how much you use the equipment. If you entire research campaign revolves around pipetting, getting it serviced after every six months or on a yearly basis is recommended.

Conclusion

Hopefully, now you will understand how the pipetting accuracy mostly depends on how you use the appliance. Implement the above-mentioned steps and reduce the margin of pipetting errors every day.

Tags

Experiments, Pipetting

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